by Staff Writers
Nagoya, Japan (SPX) Jul 31, 2017
The sorting of individual cells is necessary for many biological applications, including the isolation of specific cell types from cell suspensions. A fluorescence-activated cell sorting (FACS) has been used for high-throughput cell sorting. In this method, lasers are used to excite auto-fluorescence or tagged-fluorescence of cell included in droplets, and then droplets are diverted into different containers depending on their characteristics.
However, this technique is concerned about sample infections due to aerosols generation. Additionally, a FACS of larger cells requires the samples to be processed under low pressure through wider nozzles to prevent damage. Thus, sorting is limited to low-level throughput.
Research at Nagoya University on cell sorting used a microfluidic chip to prevent sample infection. This chip has microchannels into which cell suspensions are introduced for sorting.
The research group integrated two externally-driven on-chip pumps into the microfluidic chip for high-speed flow control. Using a high-speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds for cell sorting.
Microfluidic chip contains a cross-shaped sorting area and three-branched microfluidic channel. "Target/non-target cells are three-dimensionally aligned in the main channel," corresponding author Shinya Sakuma says.
"When target cells are detected, the on-chip pumps work rapidly to sort cells into one of two interest channels. Meanwhile, non-target cells are flushed into the waste channel without pump actuation."
The technique allows us to sort not only large but also small cells with high speed, high purity, and high viability.
We tested the method on microalgae as an example of large cells, around 100 micrometers in size, and achieved 95.8% purity, 90.8% viability, and a 92.8% success rate," corresponding co-author Yusuke Kasai says. "As a model small cell type, we used a cancer cell whose size is around 24 micrometers, and achieved 98.9% purity, 90.7% viability, and a 97.8% success rate."
The article "On-chip cell sorting by high-speed local-flow control using dual membrane pumps." was published in Lab on a Chip at DOI: 10.1039/c7lc00536a.
Washington DC (SPX) Jul 20, 2017
DARPA created the Safe Genes program to gain a fundamental understanding of how gene editing technologies function; devise means to safely, responsibly, and predictably harness them for beneficial ends; and address potential health and security concerns related to their accidental or intentional misuse. Today, DARPA announced awards to seven teams that will pursue that mission, led by: The ... read more
Hospital and Medical News at InternDaily.com
|The content herein, unless otherwise known to be public domain, are Copyright 1995-2017 - Space Media Network. All websites are published in Australia and are solely subject to Australian law and governed by Fair Use principals for news reporting and research purposes. AFP, UPI and IANS news wire stories are copyright Agence France-Presse, United Press International and Indo-Asia News Service. ESA news reports are copyright European Space Agency. All NASA sourced material is public domain. Additional copyrights may apply in whole or part to other bona fide parties. All articles labeled "by Staff Writers" include reports supplied to Space Media Network by industry news wires, PR agencies, corporate press officers and the like. Such articles are individually curated and edited by Space Media Network staff on the basis of the report's information value to our industry and professional readership. Advertising does not imply endorsement, agreement or approval of any opinions, statements or information provided by Space Media Network on any Web page published or hosted by Space Media Network. Privacy Statement|